diff --git a/django/core/validators.py b/django/core/validators.py
index 796c57e..cf5ec7a 100644
--- a/django/core/validators.py
+++ b/django/core/validators.py
@@ -89,7 +89,7 @@ class EmailValidator(object):
         r'|^"([\001-\010\013\014\016-\037!#-\[\]-\177]|\\[\001-\011\013\014\016-\177])*"$)', # quoted-string
         re.IGNORECASE)
     domain_regex = re.compile(
-        r'(?:[A-Z0-9](?:[A-Z0-9-]{0,61}[A-Z0-9])?\.)+(?:[A-Z]{2,6}|[A-Z0-9-]{2,})\.?$'  # domain
+        r'(?:[A-Z0-9](?:[A-Z0-9-]{0,61}[A-Z0-9])?\.)+(?:[A-Z]{2,6}|[A-Z0-9-]{2,})$'  # domain
         # literal form, ipv4 address (SMTP 4.1.3)
         r'|^\[(25[0-5]|2[0-4]\d|[0-1]?\d?\d)(\.(25[0-5]|2[0-4]\d|[0-1]?\d?\d)){3}\]$',
         re.IGNORECASE)
diff --git a/tests/validators/tests.py b/tests/validators/tests.py
index 367bd43..e3f418e 100644
--- a/tests/validators/tests.py
+++ b/tests/validators/tests.py
@@ -56,6 +56,7 @@ TEST_DATA = (
     # Quoted-string format (CR not allowed)
     (validate_email, '"\\\011"@here.com', None),
     (validate_email, '"\\\012"@here.com', ValidationError),
+    (validate_email, 'trailingdot@shouldfail.com.', ValidationError),
 
     (validate_slug, 'slug-ok', None),
     (validate_slug, 'longer-slug-still-ok', None),